ABSTRACT
While extracting the M. leprae from the nasal flushings of leprosy patients it was found that these organisms were trapped in the waxy layer, between the aqueous and the chloroform layers. Thin layer chromotography (TLC) analysis of this layer, using chloroform-methanol-water system, revealed different spots when sprayed with acid alcohol and heated at 160 degrees C. The TLC profile of lipids of lepromatous and borderline (MB according to the WHO terminology) leprosy patients was distinctly different from that of tuberculoid leprosy patients and normal human volunteers. A simple, economical and fast procedure to characterize patients belonging to different spectra has been developed.
Subject(s)
Chromatography, Thin Layer , Diagnosis, Differential , Humans , Leprosy/classification , Lipid Metabolism , Mycobacterium leprae/isolation & purification , Nasal Mucosa/metabolismABSTRACT
Callus cultures of Trigonella foenum-graecum were initiated from radicle or cotyledon portions of seedlings and young leaves and maintained on modified 1-B5 medium. The callus mass was disaggregated by mechanical agitation and the discrete cells thus obtained were used to measure their electrokinetic potential. Studies pertaining to the effects of ageing on electrokinetic potential and growth index revealed a relationship between these two parameters. Thus, the rate of change of electrokinotie potential with age could be employed as a parameter to study the growth kinetics of cells in callus cultures.